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Suquet, Marc; De Kermoysan, Goulwen; Araya, Ricardo Gonzalez; Queau, Isabelle; Lebrun, Luc; Le Souchu, Pierrick; Mingant, Christian. |
A reliable anaesthesia and sampling protocol for Pacific oysters will enable experiments to be conducted without sacrificing animals and will facilitate successive sampling of individuals for gametogenesis studies. As no such techniques were available for Crassostrea gigas, the present study aimed to define suitable anaesthetic conditions for use with this species. Three groups of ten oysters (mean weight +/- SD, 32.1 +/- 9.0 g) were anaesthetised in 5 L containers. Among different chemicals: benzocaine, eugenol and three different types of magnesium chloride (a laboratory one Flucka (R) - and two designed for agriculture - DEUSA International (R) and Dead SeaWorks (R)) and concentrations tested, one laboratory (concentration: 72 g L-1) and one... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Gonad sampling; Magnesium chloride; Relaxant; Anaesthesia. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6352.pdf |
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